Anti-Mucin 1 / EMA / Episialin / CD227(E29), CF594 conjugate
In Western blotting, this antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Specifications
Synonyms
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
MUC1
Gene ID
4582
NCBI Gene ID
89603
UniProt
P15941
Cellular Locus
Plasma membrane
Host
Mouse
Species Reactivity
Human
Immunogen
Delipidated extract of human milk fat globule membranes
Target Antigen
CD227 | EMA | Episialin | Mucin 1
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
E29
Conjugation
CF594
Disease
Tumor
Source
Animal
Applications
IHC, FFPE (verified)
Validated Applications
IHC,FFPE
Field of Research
Cancer, Mucins
Positive Control
MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
265-400 kDa
Additionnal Information
Shipping Conditions
Room temperature
Storage Conditions
4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
2 years
Product Datasheet
https://cdn.gentaur.com/products/37/886600/datasheet/bnc940478-100.pdf
Product MSDS
https://cdn.gentaur.com/products/37/886600/msds/bnc940478-100.pdf
CAS Number
9007-83-4
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