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Anti-Mitochondrial Marker(MTC719)

This MAb recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Not Known

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

Not Known

NCBI Gene ID

Not Known

UniProt

Not Known

Cellular Locus

Mitochondria

Host

Mouse

Species Reactivity

Multiple species,Human,Mouse,Rat

Immunogen

Mitochondrial fraction of HeLa cells

Target Antigen

Mitochondrial Marker

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

MTC719

Conjugation

Purified, BSA-free

Source

Animal

Applications

Flow, intracellular | IF (verified) | IHC, FFPE (verified)

Validated Applications

FC,IF,IHC,FFPE

Field of Research

Organelle markers

Positive Control

HeLa or HepG2 cells. Hepatic carcinomas.

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

60 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with insects or bacteria|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.25-0.5 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/886163/datasheet/bnum0719-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/886163/msds/bnum0719-50.pdf

CAS Number

9007-83-4

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