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Anti-Milk Fat Globulin(EDM45)

Recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Breast epithelial antigen BA46; EDIL1; HMFG; Lactadherin; Lactadherin precursor; Medin; MFGE8; MFGM; Milk fat globule-EGF factor 8 (MFG-E8)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

MFGE8

Gene ID

4240

NCBI Gene ID

3745

UniProt

Q08431

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Delipidated human milk fat globule membrane preparation

Target Antigen

Milk-Fat-Globulin

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

EDM45

Conjugation

Purified, with BSA

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Positive Control

Normal breast or breast carcinoma

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

45 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/885857/datasheet/bnub0942-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/885857/msds/bnub0942-500.pdf

CAS Number

9007-83-4

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