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Anti-MHC II (HLA-DP) (beta chain)(BRA-FB6), CF594 conjugate

Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linkedalpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

HLA-DPB1

Gene ID

3115

NCBI Gene ID

347270

UniProt

P04440

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Target Antigen

MHC-II

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

BRA-FB6

Conjugation

CF594

Source

Animal

Applications

Flow (verified) | Flow, surface (published) | Functional studies (published) | ELISA (published) | IP (published)

Validated Applications

FC,FC,ELISA,IP

Field of Research

Immunology, MHC antigens

Positive Control

Raji cells. Tonsil or lymph node

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

33-35 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Tissue Antigens (1988) 32(1):6-11. (IP) Cancer Sci (2008) 99(8): 1633-1642. (ELISPOT) World J Gastroenterol (2003) 9(5): 1077-1081. (Flow, surface) Eur J Immunol (2009) 39: 1947-1955. (functional studies)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/885688/datasheet/bnc940199-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/885688/msds/bnc940199-100.pdf

CAS Number

9007-83-4

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