Anti-Ku-Holo (p70;83)(KU729)
Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_
Product Specifications
Synonyms
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
XRCC6
Gene ID
2547 (p70); 7520 (p80)
NCBI Gene ID
292493 (p70); 388739 (p80)
UniProt
P12956 (p70); P13010 (p80)
Cellular Locus
Nucleus
Host
Mouse
Species Reactivity
Human,Non-human primates
Immunogen
Nuclear extract of human HL-60 cells
Target Antigen
Ku-Holo
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
KU729
Conjugation
Purified, with BSA
Source
Animal
Applications
Flow, intracellular (verified) | IF (verified)
Validated Applications
FC,IF
Field of Research
Apoptosis, Cell cycle, Signal transduction
Positive Control
Human cancer K562, HL-60, HEp-2 or HeLa cells
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
70 kDa & 80 kDa
Additionnal Information
Shipping Conditions
Room temperature
Storage Conditions
4°C; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
2 years
Product Datasheet
https://cdn.gentaur.com/products/37/884261/datasheet/bnub0729-500.pdf
Product MSDS
https://cdn.gentaur.com/products/37/884261/msds/bnub0729-500.pdf
CAS Number
9007-83-4
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