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Anti-Cytokeratin, LMW(KRTL/1077), CF740 conjugate

This MAb recognizes the 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

K1B; KRT1B; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT77

Gene ID

374454

NCBI Gene ID

334989

UniProt

Q7Z794

Cellular Locus

Cytoskeleton

Host

Mouse

Species Reactivity

Human,Rat

Immunogen

Recombinant human KRT77 protein

Target Antigen

Cytokeratin, LMW

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

KRTL/1077

Conjugation

CF740

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Cancer, Cytoskeleton

Positive Control

Skin, Squamous cell carcinoma (SCC)

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19)

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Predicted to show broad species reactivity|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/883585/datasheet/bnc741077-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/883585/msds/bnc741077-100.pdf

CAS Number

9007-83-4

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