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Anti-IL-6 (Mouse)(MP5-20F3)

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

B cell differentiation factor; B-cell stimulatory factor 2 (BSF-2); CTL differentiation factor (CDF); Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HPGF; HSF; Hybridoma growth factor Interferon beta-2; Hybridoma plasmacytoma growth factor; Interferon beta-2 (IFNB2)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

16193 (Mouse)

NCBI Gene ID

1019 (Mouse)

UniProt

P08505 (Mouse)

Cellular Locus

Secreted (extracellular)

Host

Rat

Species Reactivity

Mouse

Immunogen

Recombinant mouse IL-6 protein

Target Antigen

IL-6

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

MP5-20F3

Conjugation

Purified, BSA-free

Source

Animal

Applications

Flow, intracellular (published) | Functional studies (published)

Validated Applications

FC,Functional assays

Field of Research

Cytokines, Immunology, Inflammation

Positive Control

Mouse heart

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

21 kDa

Additionnal Information

Flow cytometry: 0.5-1 ug/million cells in 0.1 mL|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with human or rat; others not known|Neutralization Studies order Ab without azide|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (1990) 145(12): 4185-4191. (functional studies) J Immunol (2013) 190:703-711. (functional studies; Flow)

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/883541/datasheet/bnum0663-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/883541/msds/bnum0663-50.pdf

CAS Number

9007-83-4

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