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Anti-IgA Secretory Component(SC05), CF405S conjugate

This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ECM1

Gene ID

1893

NCBI Gene ID

81071

UniProt

Q16610

Cellular Locus

Secreted (extracellular)

Host

Mouse

Species Reactivity

Human,Rat

Immunogen

Secretory Component protein isolated from human colostrum

Target Antigen

IgA Secretory Component

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

SC05

Conjugation

CF405S

Source

Animal

Applications

Functional studies (published) | IHC, FFPE (verified) | WB (published)

Validated Applications

IHC,FFPE,WB,Functional assays

Field of Research

Cancer, Immunology, Inflammation

Positive Control

Stomach, Lung, or breast tumor

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

~80 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Infection Immunity (2010)78(11): 4589-4592. (WB) Clin Exp Immunol (2017) 195: 287-301. (functional studies)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/883093/datasheet/bnc040050-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/883093/msds/bnc040050-500.pdf

CAS Number

9007-83-4

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