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Anti-Human Nuclear Antigen(235-1), CF647 conjugate

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

HNA; HuNu

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

Not Known

NCBI Gene ID

Not Known

UniProt

Not Known

Cellular Locus

Nucleus

Host

Mouse

Species Reactivity

Human,Non-human primates

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Target Antigen

Human Nuclear Antigen

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

235-1

Conjugation

CF647

Source

Animal

Applications

Flow, intracellular (verified) | IF (verified) | IHC, frozen (verified)

Validated Applications

FC,IF,IHC,

Field of Research

Organelle markers

Positive Control

All human cells. Tonsil

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

70 kDa & 80 kDa

Additionnal Information

Immunohistology Frozen 0.5-1.0 ug/mL|Immunocytochemistry acetone-fixed cells 0.25-0.5 ug/mL|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunoprecipitation 0.5-1 ug/500 ug protein lysate|Does not react with mouse, rat or chicken; others not known|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/882803/datasheet/bnc470099-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/882803/msds/bnc470099-500.pdf

CAS Number

9007-83-4

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