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Anti-HSP60(HSPD1/875)

Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (HSPs), more appropriately called as stress response proteins (SRPs). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

60kDa chaperonin; 60kDa heat shock protein mitochondrial; Chaperonin; 60-KD (CPN60); GROEL; HLD4; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (SPG13)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

HSPD1

Gene ID

3329

NCBI Gene ID

595053

UniProt

P10809

Cellular Locus

Mitochondria

Host

Mouse

Species Reactivity

Chicken,Guinea pig,Hamster,Human,Monkey,Mouse,Pig,Rat

Immunogen

Recombinant human HSPD1 protein

Target Antigen

HSP60

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

HSPD1/875

Conjugation

Purified, BSA-free

Source

Animal

Applications

IF (verified) | IHC, FFPE (verified)

Validated Applications

IF,IHC,FFPE

Field of Research

Autoimmunity, Heat shock proteins, Immunology, Inflammation

Positive Control

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

60 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/882667/datasheet/bnum0875-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/882667/msds/bnum0875-50.pdf

CAS Number

9007-83-4

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