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Anti-HSP27(HSPB1/774), Biotin conjugate

This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Heat shock 27kDa protein; Estrogen-regulated 24kDa protein; Heat shock 25kDa protein 1; Heat shock 28kDa protein 1; Heat shock protein beta-1; HMN2B; Hsp25; Hsp28; HspB1; Stress-responsive protein 27 (SRP27)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

HSPB1

Gene ID

3315

NCBI Gene ID

520973

UniProt

P04792

Cellular Locus

Cytoplasmic|Plasma membrane

Host

Mouse

Species Reactivity

Chicken,Chimpanzee,Human,Mouse,Rat,Sheep

Immunogen

Recombinant human HSPB1 protein

Target Antigen

HSP27

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

HSPB1/774

Conjugation

Biotin

Source

Animal

Applications

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)

Validated Applications

FC,IF,IHC,FFPE,WB

Field of Research

Endocrinology, Heat shock proteins

Positive Control

~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

27 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.25-0.5 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/882557/datasheet/bncb0774-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/882557/msds/bncb0774-500.pdf

CAS Number

9007-83-4

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