Anti-HSP27(HSPB1/774), Biotin conjugate
This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
Product Specifications
Synonyms
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
HSPB1
Gene ID
3315
NCBI Gene ID
520973
UniProt
P04792
Cellular Locus
Cytoplasmic|Plasma membrane
Host
Mouse
Species Reactivity
Chicken,Chimpanzee,Human,Mouse,Rat,Sheep
Immunogen
Recombinant human HSPB1 protein
Target Antigen
HSP27
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
HSPB1/774
Conjugation
Biotin
Source
Animal
Applications
Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
Validated Applications
FC,IF,IHC,FFPE,WB
Field of Research
Endocrinology, Heat shock proteins
Positive Control
~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
27 kDa
Additionnal Information
Shipping Conditions
Room temperature
Storage Conditions
4°C; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
2 years
Product Datasheet
https://cdn.gentaur.com/products/37/882557/datasheet/bncb0774-500.pdf
Product MSDS
https://cdn.gentaur.com/products/37/882557/msds/bncb0774-500.pdf
CAS Number
9007-83-4
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