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Anti-GM-CSF(BVD2-21C11)

Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Burst Promoting Activity; Eosinophil Colony Stimulating Factor; Molgramostin; Pluripoietin Alpha; Sargramostim

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CSF2

Gene ID

1437

NCBI Gene ID

1349

UniProt

P04141

Cellular Locus

Secreted (extracellular)

Host

Rat

Species Reactivity

Cynomolgus,Human,Rhesus macaque

Immunogen

Recombinant human GM-CSF protein

Target Antigen

GM-CSF

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

BVD2-21C11

Conjugation

Purified, with BSA

Source

Animal

Applications

Flow, intracellular (published) | IF (published) | ELISA (published)

Validated Applications

FC,IF,ELISA

Field of Research

Cytokines, Immunology

Positive Control

Lymph node and tonsil

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

22 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunoprecipitation: 0.5-1 ug/500 ug protein lysate|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (frozen) 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Neutralization Studies order Ab without azide|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (2015) 194: 5085-5093. (Flow, intracellular; IF) J Exp Med (1999) 190 (6): 875-880. (ELISA)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/880994/datasheet/bnub0662-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/880994/msds/bnub0662-100.pdf

CAS Number

9007-83-4

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