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Anti-GFAP(GA-5), CF405S conjugate

This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

GFAP

Gene ID

2670

NCBI Gene ID

514227

UniProt

P14136

Cellular Locus

Cytoskeleton

Host

Mouse

Species Reactivity

Chicken,Cow,Human,Mouse,Pig,Rabbit,Rat

Immunogen

GFAP isolated from pig spinal cord

Target Antigen

GFAP

Clonality

Monoclonal

Isotype

IgG1

Clone

GA-5

Conjugation

CF405S

Source

Animal

Applications

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)

Validated Applications

FC,IF,IHC,FFPE,WB

Field of Research

Neuroscience

Positive Control

Brain or Astrocytoma

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

~50 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/880586/datasheet/bnc040167-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/880586/msds/bnc040167-100.pdf

CAS Number

9007-83-4

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