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Anti-Ep-CAM / CD326(323/A3), Biotin conjugate

EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

TACSTD1

Gene ID

4072

NCBI Gene ID

542050

UniProt

P16422

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

MCF-7 human breast cancer cells

Target Antigen

CD326 | Ep-CAM

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

323/A3

Conjugation

Biotin

Disease

Tumor

Source

Animal

Applications

Flow (verified) | IHC, FFPE (verified)

Validated Applications

FC,IHC,FFPE

Field of Research

Cancer

Positive Control

HT29 cells or breast tumor

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

40-43 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with rat; others not known|Immunohistology formalin-fixed 0.5-1.0 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/879479/datasheet/bncb0396-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/879479/msds/bncb0396-500.pdf

CAS Number

9007-83-4

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