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Anti-Cytokeratin, multi(C11), CF405S conjugate

This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT4

Gene ID

3851 (CK4); 3852 (CK5); 3853 (CK6A); 3854 (CK6B); 286887 (CK6C); 3856 (CK8); 3858 (CK10); 3860 (CK13); 3875 (CK18)

UniProt

P19013 (CK4); P13647 (CK5); P02538 (CK6A); P04259 (CK6B); P48668 (CK6C); P05787 (CK8); P13645 (CK10); P13646 (CK13); P05783 (CK18)

Cellular Locus

Cytoskeleton

Host

Mouse

Species Reactivity

Cow,Frog,Goat,Guinea pig,Human,Marmoset,Mouse,Pig,Rat

Immunogen

Keratin-enriched preparation from cultured human A431

Target Antigen

Cytokeratin, multi

Clonality

Monoclonal

Isotype

IgG1

Clone

C11

Conjugation

CF405S

Source

Animal

Applications

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (published)

Validated Applications

FC,IF,IHC,FFPE,WB

Field of Research

Cancer, Cytoskeleton

Positive Control

A431 cells, Skin, Colon carcinoma

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

Multiple

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1.0ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Western blotting 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Cancer Res (2012) 18(4): 993-1003. (IHC, FFPE; western)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/878723/datasheet/bnc040393-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/878723/msds/bnc040393-500.pdf

CAS Number

9007-83-4

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