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Anti-Cytokeratin 5/8(C-50), Biotin conjugate

This antibody reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

CK5; DDD; EBS2; Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; K5; Keratin complex 2, basic, Gene 5; Keratin Type II Cytoskeletal 5; Keratin-5; KRT5; KRT5A; Type II Cytoskeletal 5; Type-II Keratin Kb5. CK8; CARD2; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT5

Gene ID

3852 (CK5); 3856 (CK8)

NCBI Gene ID

433845 (CK5); 533782; 708445 (CK8)

UniProt

P13647 (CK5); P05787 (CK8)

Cellular Locus

Cytoskeleton

Host

Mouse

Species Reactivity

Cow,Dog,Ferret,Hamster,Human,Mouse,Pig,Rat,Sheep

Immunogen

Cytoskeletal preparation from HeLa cells

Target Antigen

Cytokeratin 5/8

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

C-50

Conjugation

Biotin

Disease

Tumor

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Cancer, Cytoskeleton

Positive Control

HepG2 or A431 cells. Skin or Squamous cell carcinoma

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

58 kDa (CK5); 52.5 kDa (CK8)

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1.0 ug/mL|For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/mL PBS, 15 min at RT|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Western blotting 0.5-1.0 ug/mL|Does not react with chicken , or Xenopus|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/877650/datasheet/bncb0948-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/877650/msds/bncb0948-100.pdf

CAS Number

9007-83-4

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