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Anti-Cytokeratin 18(DA7)

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18 Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT18

Gene ID

3875

NCBI Gene ID

406013

UniProt

P05783

Cellular Locus

Cytoskeleton

Host

Mouse

Species Reactivity

Human

Immunogen

Human breast cancer PMC 42 cells

Target Antigen

Cytokeratin 18

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

DA7

Conjugation

Purified, with BSA

Disease

Tumor

Source

Animal

Applications

IF (verified) | IHC, FFPE (verified) | WB (verified)

Validated Applications

IF,IHC,FFPE,WB

Field of Research

Cancer, Cytoskeleton

Positive Control

MCF-7, HeLa cells, Breast Cancer

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

45 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Does not react with mouse, rat, hamster, cow, dog, pig, or sheep; others not known|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/877111/datasheet/bnub0198-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/877111/msds/bnub0198-500.pdf

CAS Number

9007-83-4

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