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Anti-Cytokeratin 10/13(DE-K13), CF647 conjugate

This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT10

Gene ID

3858

NCBI Gene ID

99936

UniProt

P13645

Cellular Locus

Cytoskeleton

Host

Mouse

Species Reactivity

Cat,Human

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium

Target Antigen

Cytokeratin 10/13

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

DE-K13

Conjugation

CF647

Disease

Tumor

Source

Animal

Applications

IF (verified) | IHC, FFPE (verified)

Validated Applications

IF,IHC,FFPE

Field of Research

Cancer, Cytoskeleton

Positive Control

A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

56.5 kDa (CK10) & 53 kDa (CK13)

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.25-0.5 ug/mL

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/876908/datasheet/bnc470696-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/876908/msds/bnc470696-100.pdf

CAS Number

9007-83-4

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