Anti-Cytokeratin 10/13(DE-K13)
This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Specifications
Synonyms
BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT1
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
KRT10
Gene ID
3858
NCBI Gene ID
99936
UniProt
P13645
Cellular Locus
Cytoskeleton
Host
Mouse
Species Reactivity
Cat,Human
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium
Target Antigen
Cytokeratin 10/13
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
DE-K13
Conjugation
Purified, BSA-free
Disease
Tumor
Source
Animal
Applications
IF (verified) | IHC, FFPE (verified)
Validated Applications
IF,IHC,FFPE
Field of Research
Cancer, Cytoskeleton
Positive Control
A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
Concentration
1 mg/mL
Buffer
PBS, no BSA, no azide
Molecular Weight
56.5 kDa (CK10) & 53 kDa (CK13)
Additionnal Information
Shipping Conditions
Room temperature
Storage Conditions
-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
2 years
Product Datasheet
https://cdn.gentaur.com/products/37/876885/datasheet/bnum0696-50.pdf
Product MSDS
https://cdn.gentaur.com/products/37/876885/msds/bnum0696-50.pdf
CAS Number
9007-83-4
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