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Anti-Creatine Phosphokinase(2ba6), CF405S conjugate

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments. They provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteinsPrimary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

B-CK; BB-CK; creatine kinase-B; Creatine kinase B chain; CKBB creatine kinase B-type; creatine kinase; brain; CKBB; Brain creatine kinase; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine phosphokinase BB

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CKB

Gene ID

1152

NCBI Gene ID

173724

UniProt

P12277

Cellular Locus

Cytoplasmic

Host

Mouse

Species Reactivity

Human

Immunogen

Human CKBB protein

Target Antigen

Creatine Phosphokinase

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

2ba6

Conjugation

CF405S

Source

Animal

Field of Research

Metabolism

Positive Control

Cerebellum

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

43 kDa (Monomer); 86 kDa (Dimer)

Additionnal Information

For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Not suitable for FFPE sections|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/876514/datasheet/bnc040196-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/876514/msds/bnc040196-100.pdf

CAS Number

9007-83-4

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