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Anti-CDw75(LN-1)

Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin's disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues._x000D__x000D_This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

Product Specifications

Synonyms

6-sialyltransferase 1 (6-ST1) | Alpha 2,6 ST1 | Beta galactoside alpha 2,6 sialyltransferase 1 | CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 | CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 | SIAT1 | ST6 beta galactosamide alpha 2,6 sialyltranferase 1 | ST6Gal I | ST6N

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ST6GAL1

Gene ID

6480

NCBI Gene ID

207459

UniProt

P15907

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

Target Antigen

CDw75

Clonality

Monoclonal

Isotype

IgM κ

Clone

LN-1

Conjugation

Purified, with BSA

Disease

Tumor

Source

Animal

Applications

Flow, surface (verified) | IF (verified) | IHC, FFPE (verified)

Validated Applications

FC,IF,IHC,FFPE

Field of Research

Immunology

Positive Control

HeLa or Daudi Cells. Germinal center B-cells in a lymph node or tonsil. Use LN1 s ability to stain surface of erythrocytes as an internal positive control.

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

46 kDa

Additionnal Information

Immunohistology (formalin)|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/875553/datasheet/bnub0055-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/875553/msds/bnub0055-500.pdf

CAS Number

9007-83-4

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