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Anti-CD98(UM7F8), CF594 conjugate

CD98 exits as a heterodimer containing a disulphide-linked glycosylated heavy chain and a non-glycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

4F2, 4F2HC, 4T2HC, CD98HC; MDU1; NACAE; Slc3a2; Solute Carrier Family 3 (activators of dibasic and neutral amino acid transport) Member 2, E16, LAT1, MPE16, hLAT1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SLC3A2

Gene ID

6520

NCBI Gene ID

502769

UniProt

P08195

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Molt 13 T cell line

Target Antigen

CD98

Clonality

Monoclonal

Isotype

IgG1

Clone

UM7F8

Conjugation

CF594

Source

Animal

Applications

Flow, surface (published) | IF (published) | Functional studies (published) | IP (published)

Validated Applications

FC,IF,IP

Field of Research

Transporters

Positive Control

HL-60, Jurkat, MG63, HUT-78, K562, YT, U937, Hep-G2 cells, Tonsil

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

Heterodimer (125 kDa) made of a disulfide-linked glycosylated heavy chain of approximately 80-90 kDa and a non-glycosylated light chain

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Comitogenic with soluble anti-CD2 and immobilized anti-CD3 mAbs|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Cell Immunol (1994) 154(1):253-63. (IP, Flow, functional studies) J Cell Biol (2001) 276(42): 39282-39289. (IF) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 280-283. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 531-534.

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/875194/datasheet/bnc940425-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/875194/msds/bnc940425-100.pdf

CAS Number

9007-83-4

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