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Anti-CD90(AF-9)

Recognizes a protein of 18-35 kDa, identified as CD90 (also known as Thy1). CD90 is a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34 cells in bone marrow, and Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

THY1

Gene ID

7070

NCBI Gene ID

644697

UniProt

P04216

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Cow,Human

Immunogen

Human T-lymphoma cells

Target Antigen

CD90 | Thy1

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AF-9

Conjugation

Purified, BSA-free

Source

Animal

Applications

Flow, surface (published) | IF (published) | IHC, FFPE (published)

Validated Applications

FC,IF,IHC,FFPE

Field of Research

Cell adhesion, Immunology

Positive Control

IMR-32, CCRF-CEM or MOLT-4 cells. Human uterus.

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

18-35 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Int J Stem Cell Res Ther (2018) 5:056. (IHC, FFPE) Biology Open 2017 6: 1423-1433. (Flow) Gastroenterol (2009) 136(2): P705-714. (IF)

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/875023/datasheet/bnum0987-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/875023/msds/bnum0987-50.pdf

CAS Number

9007-83-4

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