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Anti-CD84(152-1D5)

Recognizes a protein of 74 kDa, identified as CD84 (Workshop V; Code A085, C057). It is expressed on mature B cells and on B-cell lines, including pre-B-cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD84

Gene ID

8832

NCBI Gene ID

398093

UniProt

Q9UIB8

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Spleen cells of a patient with hairy cell leukemia

Target Antigen

CD84

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

152-1D5

Conjugation

Purified, BSA-free

Source

Animal

Applications

Flow (verified) | Flow, surface (published) | Functional studies (published) | IP (published) | WB (published)

Validated Applications

FC,FC,IP,WB

Field of Research

Immunology

Positive Control

Raji, HL60, THP-1, HMC-1, HEL and NAMALWA cells. Human platelets and macrophages. Human tonsil.

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

74 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, Oxford University Press, Oxford, 1995. Blood (1997) 90(6): 2398-2405. (Flow, surface; IP) Eur J Immunol (2002) 32: 1640-1649. (Flow, surface; IP; WB) Oncogene (2014) 33: 1006-1016. (functional studies; Flow; IP)

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/874833/datasheet/bnum0985-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/874833/msds/bnum0985-50.pdf

CAS Number

9007-83-4

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