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Anti-CD57 / B3GAT1(NK-1)

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA._x000D_ _x000D_ This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

Product Specifications

Synonyms

3-Glucuronyltransferase 1; B3GAT1; Galactosylgalactosylxylosylprotein 3-beta-Glucuronosyltransferase 1; GLCATP; GlcUAT-P; Glucuronosyltransferase P; UDP GlcUA Glycoprotein beta 1, 3 Glucuronyltransferase

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

B3GAT1

Gene ID

27087

NCBI Gene ID

381050

UniProt

Q9P2W7

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Human peripheral blood mononuclear cells

Target Antigen

B3GAT1 | CD57

Clonality

Monoclonal

Isotype

IgM κ

Clone

NK-1

Conjugation

Purified, with BSA

Source

Animal

Applications

Flow, surface (published) | IF (published) | IHC, FFPE (verified) | WB (published)

Validated Applications

FC,IF,IHC,FFPE,WB

Field of Research

Cancer, Immunology

Positive Control

Lymph node or Tonsil

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

~110 kDa (Glycoprotein)

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with rat; others not known|Immunohistology formalin-fixed 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Asian J Androl (2010) 12(4): 548-555. (WB blot; Flow) Cancer Immunol Immunother (2011) 60:1683-1695. (Flow, surface) Cancer Sci (2016) 107: 846-852. (IHC, FFPE) Cell Stem Cell (2017) 20(6): 874-890.e7. (IF; Flow, surface)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/872856/datasheet/bnub0016-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/872856/msds/bnub0016-100.pdf

CAS Number

9007-83-4

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