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Anti-CD50 / ICAM3(ICO-60)

Recognizes the N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C.

Product Specifications

Synonyms

ICAMR; Intercellular adhesion molecule 3 (ICAM3)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ICAM3

Gene ID

3385

NCBI Gene ID

354563

UniProt

P32942

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Human ICAM3

Target Antigen

CD50

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

ICO-60

Conjugation

Purified, BSA-free

Source

Animal

Applications

Flow, surface (published) | Functional studies (published)

Validated Applications

FC,Functional assays

Field of Research

Immunology

Positive Control

HL-60 or THP-1 cells. Lymph node and tonsil

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

110-160 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Gen Virol (1995) 76: 1345-1352. (functional assay) JBC (1996) 271(39): 23920-23927. (Flow, functional assay)

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/872325/datasheet/bnum1040-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/872325/msds/bnum1040-50.pdf

CAS Number

9007-83-4

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