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Anti-CD48(156-4H9)

Reacts with human CD48, a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

B-cell activation marker|B-lymphocyte activation marker BLAST-1|BCM1 surface antigen|BLAST1|CD48 antigen (B cell membrane protein)|Signaling lymphocytic activation molecule 2 (SLAMF2)|SLAM family member 2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD48

Gene ID

962

NCBI Gene ID

243564

UniProt

P09326

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Human CD48 protein

Target Antigen

CD48

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

156-4H9

Conjugation

Purified, with BSA

Source

Animal

Applications

Flow, surface (published) | Functional studies (published) | IF (published)

Validated Applications

FC,IF

Field of Research

Immunology

Positive Control

Daudi, JY, Raji, Jurkat, and human lymphocytes. Human lymph node and tonsil.

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

45 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Arthritis Res Ther (2006) 8, R88. (functional studies, Flow) PLoS ONE (2017) 12(10), e0185940. (Flow, IF)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/871944/datasheet/bnub0307-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/871944/msds/bnub0307-100.pdf

CAS Number

9007-83-4

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