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Anti-CD47(IAP/964), CF647 conjugate

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Antigenic Surface Determinant Protein OA3; IAP; Integrin Associated Protein; Integrin Associated Signal Transducer; MER6; OA3; Protein MER6; Rh Related Antigen

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD47

Gene ID

961

NCBI Gene ID

446414

UniProt

Q08722

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human,Mouse

Immunogen

Recombinant human IAP protein

Target Antigen

CD47

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

IAP/964

Conjugation

CF647

Source

Animal

Applications

Flow (verified) | IF (verified) | IHC, FFPE (verified)

Validated Applications

FC,IF,IHC,FFPE

Field of Research

Cell adhesion, Hematology, Neuroscience, Signal transduction

Positive Control

HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

~50 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/871929/datasheet/bnc470964-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/871929/msds/bnc470964-500.pdf

CAS Number

9007-83-4

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