Anti-CD45RO(UCHL-1)
Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
Product Specifications
Synonyms
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
PTPRC
Gene ID
5788
NCBI Gene ID
654514
UniProt
P08575
Cellular Locus
Plasma membrane
Host
Mouse
Species Reactivity
Chimpanzee,Human,Marmoset
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1); Recombinant human CD45RO protein (T200/797)
Target Antigen
CD45RO
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
UCHL-1
Conjugation
Purified, BSA-free
Disease
Tumor
Source
Animal
Applications
Functional studies (published) | Flow, surface (verified) | IF (verified) | IHC, FFPE (verified) | IP (published) | WB (published)
Validated Applications
FC,IF,IHC,FFPE,IP,WB,Functional assays
Field of Research
Immunology, Signal transduction
Positive Control
CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Concentration
1 mg/mL
Buffer
PBS, no BSA, no azide
Molecular Weight
180-185 kDa
Additionnal Information
References & Citations
Shipping Conditions
Room temperature
Storage Conditions
-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
2 years
Product Datasheet
https://cdn.gentaur.com/products/37/871679/datasheet/bnum0003-50.pdf
Product MSDS
https://cdn.gentaur.com/products/37/871679/msds/bnum0003-50.pdf
CAS Number
9007-83-4
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