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Anti-CD15 / FUT4 / Lewis x(FR4A5)

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on more than 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin'€™s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance._x000D_ _x000D_ Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_ This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

Product Specifications

Synonyms

3 Fucosyl N Acetyl Lactosamine; Alpha (1,3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FUT4

Gene ID

2526

NCBI Gene ID

654379

UniProt

P22083

Cellular Locus

Plasma membrane|Perinuclear

Host

Mouse

Species Reactivity

Human

Immunogen

Myelomonocytic leukemia cells

Target Antigen

CD15 | FUT4 | Lewis-x

Clonality

Monoclonal

Isotype

IgM κ

Clone

FR4A5

Conjugation

Purified, with BSA

Disease

Tumor

Source

Animal

Applications

Flow (verified) | IF (verified)

Validated Applications

FC,IF

Field of Research

Immunology

Positive Control

U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

~220 kDa (Glycoprotein)

Additionnal Information

Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/867308/datasheet/bnub0504-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/867308/msds/bnub0504-100.pdf

CAS Number

9007-83-4

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