Anti-CD15 / FUT4 / Lewis x(FR4A5)
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on more than 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin'€™s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance._x000D_ _x000D_ Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_ This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.
Product Specifications
Synonyms
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
FUT4
Gene ID
2526
NCBI Gene ID
654379
UniProt
P22083
Cellular Locus
Plasma membrane|Perinuclear
Host
Mouse
Species Reactivity
Human
Immunogen
Myelomonocytic leukemia cells
Target Antigen
CD15 | FUT4 | Lewis-x
Clonality
Monoclonal
Isotype
IgM κ
Clone
FR4A5
Conjugation
Purified, with BSA
Disease
Tumor
Source
Animal
Applications
Flow (verified) | IF (verified)
Validated Applications
FC,IF
Field of Research
Immunology
Positive Control
U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
~220 kDa (Glycoprotein)
Additionnal Information
Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Shipping Conditions
Room temperature
Storage Conditions
4°C; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
2 years
Product Datasheet
https://cdn.gentaur.com/products/37/867308/datasheet/bnub0504-100.pdf
Product MSDS
https://cdn.gentaur.com/products/37/867308/msds/bnub0504-100.pdf
CAS Number
9007-83-4
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