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Anti-CD11c(HC1/1), CF647 conjugate

CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells. It induces cellular activation and helps trigger neutrophil respiratory burst. It is expressed in hairy cell leukemias, acute non-lymphocytic leukemias, and some B-cell chronic lymphocytic leukemias._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

CD11 antigen-like family member B|CD11b/CD18|CD49d|Cell surface glycoprotein MAC-1 subunit alpha|Complement Component Receptor 3 Alpha|CR3 Alpha Chain (CR3A)|Integrin alpha-M|Integrin beta 2 alpha subunit|ITGAM|Leukocyte adhesion receptor MO1; Ly-40|Mac-1a|MAC1|Mac1, alpha subunit|MAC1A|Macrophage antigen alpha polypeptide; MO1A|Neutrophil adherence receptor alpha M subunit

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ITGAX

Gene ID

3687

NCBI Gene ID

248472

UniProt

P20702

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Human monocytic U937 phorbol ester-treated cells

Target Antigen

CD11c

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

HC1/1

Conjugation

CF647

Disease

Tumor

Source

Animal

Applications

Flow (verified) | Flow, surface (published) | Functional studies (published) | IP (published)

Validated Applications

FC,FC,IP

Field of Research

Cell adhesion, Immunology

Positive Control

Human dendritic cells. Human lymph nodes and tonsils.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

145 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Hybridoma (1988) 7:2, 167-176. (IHC) J Exp Med (1991) 174 (6): 1313-1322. (Flow; receptor activation; IP)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/866837/datasheet/bnc470152-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/866837/msds/bnc470152-500.pdf

CAS Number

9007-83-4

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