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Anti-Carbonic Anhydrase IX(CA9/781)

Carbonic anhydrase IX (carbonic anhydrase 9) is one of several carbolic anhydrases that vary in tissue distribution and localization. Carbonic anhydrases catalyze the interconversion of carbon dioxide and water into carbonic acid and bicarbonate and are found in all mammalian tissues. Carbonic anhydrase IX is a transmembrane protein that is not expressed in most healthy tissues, but is expressed in most renal cell carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Carbonic anhydrase IX; Carbonic anhydrase 9; RCC-associated antigen G250; Carbonic dehydratase; CA-IX; Membrane antigen MN

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CA9

Gene ID

768

NCBI Gene ID

63287

UniProt

Q16790

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Horse,Human

Immunogen

Recombinant human CA9 protein

Target Antigen

Carbonic Anhydrase IX

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CA9/781

Conjugation

Purified, with BSA

Disease

Tumor

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Cancer, Metabolism

Positive Control

Normal kidney or renal cell carcinoma

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

55 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/866167/datasheet/bnub0781-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/866167/msds/bnub0781-500.pdf

CAS Number

9007-83-4

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