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Anti-Calprotectin (MRP14)(S100A9)(CAGB/426)

Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B). This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

Product Specifications

Synonyms

Calprotectin; Protein S100-A9; Myeloid-related protein 14; MRP-14; Calgranulin B; CAGB; Calprotectin L1H Subunit

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

S100A9

Gene ID

6280 (S100A9 / Calgranulin B / MRP-14)

NCBI Gene ID

112405 (S100A9 / Calgranulin B / MRP-14)

UniProt

P06702 (S100A9 / Calgranulin B / MRP-14)

Cellular Locus

Cytoplasmic|Cytoskeleton

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant human CAGB protein

Target Antigen

Calprotectin | S100-A9

Clonality

Monoclonal

Isotype

IgM κ

Clone

CAGB/426

Conjugation

Purified, with BSA

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Immunology, Inflammation

Positive Control

Tonsil, lymph node, or spleen

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

14 kDa

Additionnal Information

Immunohistology formalin-fixed. Not suitable for frozen tissues. 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/865977/datasheet/bnub0426-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/865977/msds/bnub0426-500.pdf

CAS Number

9007-83-4

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