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Anti-Calgranulin B / MRP-8/-14 / MAC 387 / S100A8/A9 / Macrophage Ag(MAC387)

Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B). This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Calprotectin; Protein S100-A9; Myeloid-related protein 14; MRP-14; Calgranulin B; CAGB; Calprotectin L1H Subunit

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

6279 (S100A8/Calgranulin A/MRP-8) & 6280 (S100A9/Calgranulin B/MRP-14)

NCBI Gene ID

416073 (S100A8/Calgranulin A/MRP-8) & 112405 (S100A9/Calgranulin B/MRP-14)

UniProt

P05109 (S100A8/Calgranulin A/MRP-8); P06702 (S100A9/Calgranulin B/MRP-14)

Cellular Locus

Cytoplasmic|Cytoskeleton

Host

Mouse

Species Reactivity

Baboon,Cat,Cow,Dog,Goat,Guinea pig,Horse,Human,Monkey,Mouse,Pig,Rabbit,Rat

Immunogen

Affinity purified monocyte membrane preparation

Target Antigen

Calprotectin | S100-A9

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

MAC387

Conjugation

Purified, BSA-free

Source

Animal

Applications

Flow, intracellular (published) | IHC, FFPE (verified) | IP (published) | WB (published)

Validated Applications

FC,IHC,FFPE,IP,WB

Field of Research

Immunology, Inflammation

Positive Control

Tonsil, lymph node, or spleen

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

12-14 kDa (doublet)

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Histochem Cytochem (1987) 35(11): 1217-1226 (IP; WB; IHC, FFPE) J Histochem Cytochem (1990) 38(11): 1669-1675. (IHC, FFPE) J Leukocyte Biol (1994) 55: 259. (WB; IHC) World J Gastroenterol (2017)23(8): 1477-1488. (Flow, intracellular)

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/865941/datasheet/bnum0035-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/865941/msds/bnum0035-50.pdf

CAS Number

9007-83-4

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