Anti-BrdU (Bromodeoxyuridine)(BRD494)
Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA. This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).
Product Specifications
Synonyms
Bromodeoxyuridine; 5-bromo-2-deoxyuridine
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
Not Applicable
UniProt
Not Applicable
Cellular Locus
Nucleus
Host
Mouse
Species Reactivity
Pan species
Immunogen
Bromodeoxyuridine (BrdU) conjugated to KLH
Target Antigen
BrdU
Clonality
Monoclonal
Isotype
IgG1
Clone
BRD494
Conjugation
Purified, BSA-free
Source
Animal
Applications
IHC, FFPE (verified)
Validated Applications
IHC,FFPE
Field of Research
Cancer, Cell cycle
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU
Concentration
1 mg/mL
Buffer
PBS, no BSA, no azide
Molecular Weight
Depends on the target
Additionnal Information
Immunohistology formalin-fixed 0.5-1 ug/mL|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL
Shipping Conditions
Room temperature
Storage Conditions
-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
2 years
Product Datasheet
https://cdn.gentaur.com/products/37/865780/datasheet/bnum0494-50.pdf
Product MSDS
https://cdn.gentaur.com/products/37/865780/msds/bnum0494-50.pdf
CAS Number
9007-83-4
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