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Anti-BrdU (Bromodeoxyuridine)(BRD469 + BRD494 + BRD.3)

Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA. This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).

Product Specifications

Synonyms

Bromodeoxyuridine; 5-bromo-2-deoxyuridine

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

Not Applicable

UniProt

Not Applicable

Cellular Locus

Nucleus

Host

Mouse

Species Reactivity

Pan species

Immunogen

Bromodeoxyuridine (BrdU) conjugated to BSA or KLH

Target Antigen

BrdU

Clonality

Monoclonal

Isotype

IgG1

Clone

BRD469 BRD494 BRD.3

Conjugation

Purified, with BSA

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Cancer, Cell cycle

Positive Control

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

Depends on the target

Additionnal Information

Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/mL PBS, 10 min at 37C|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/865772/datasheet/bnub1000-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/865772/msds/bnub1000-500.pdf

CAS Number

9007-83-4

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