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Anti-Beta-2-Microglobulin (B2M)(246-E9.E7), CF568 conjugate

Beta-2 microglobulin is a component of the class I major histocompatibility complex (MHC I) where it functions in antigen processing and presentation. Involved in the presentation of peptide antigens to the immune system. Human Beta-2 microglobulin is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This MAb is specific to human Beta-2 microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. The detection of Beta-2 microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection. Clone 246 E9.E7 is the same as HLA.ABC.m2.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

B2M; Beta 2 microglobulin

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

B2M

Gene ID

567

NCBI Gene ID

534255

UniProt

P61769

Cellular Locus

Endoplasmic reticulum|Plasma membrane|Secreted (extracellular)

Host

Mouse

Species Reactivity

Human

Immunogen

Human PBLs from a T-cell acute lymphoblastic leukemia (T-ALL) patient

Target Antigen

Beta-2 Microglobulin

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

246-E9.E7|same as HLA.ABC.m2

Conjugation

CF568

Source

Animal

Applications

Flow, surface (published) | IF (published) | Functional studies (published) | IP (published)

Validated Applications

FC,IF,IP

Field of Research

Cancer, Immunology, Inflammation

Positive Control

HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

12 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with non-human primates; others not known|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Histochem Cytochem (1985) 33(12): 1183-1189. (IF) Immunol Cell Biol (1988) 66 (Pt 1) 69-77. (platelet aggregation) Br J Cancer (1992) 66: 88-92. (IP)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/865443/datasheet/bnc681039-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/865443/msds/bnc681039-100.pdf

CAS Number

9007-83-4

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