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Anti-bcl-x(BX006), CF568 conjugate

Bcl-x belongs to the Bcl-2 family of proteins and plays a crucial role in apoptosis. Bcl-X has two isoforms: Bcl-XL (long), a 26 kDa protein which suppresses cell death, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2 and promotes apoptosis. This MAb reacts with both Bcl-XS and Bcl-XL proteins, and shows no cross-reaction with Bcl-2 or Bax proteins.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Bcl-x; Bcl-2-like protein 1; BCL2L1; Apoptosis regulator Bcl-X

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

BCL2L1

Gene ID

598

NCBI Gene ID

516966

UniProt

Q07817

Cellular Locus

Cytoplasmic|Endoplasmic reticulum|Mitochondria

Host

Mouse

Species Reactivity

Human,Mouse,Pig,Rat

Immunogen

A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.

Target Antigen

Bcl-x

Clonality

Monoclonal

Isotype

IgG2a

Clone

BX006

Conjugation

CF568

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Apoptosis, Cancer

Positive Control

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

27 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/865291/datasheet/bnc680006-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/865291/msds/bnc680006-100.pdf

CAS Number

9007-83-4

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