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Anti-bcl-x(2H12)

Bcl-x belongs to the Bcl-2 family of proteins and plays a crucial role in apoptosis. Bcl-X has two isoforms: Bcl-XL (long), a 26 kDa protein which suppresses cell death, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2 and promotes apoptosis. This MAb reacts with both Bcl-XS and Bcl-XL proteins, and shows no cross-reaction with Bcl-2 or Bax proteins._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Bcl-x; Bcl-2-like protein 1; BCL2L1; Apoptosis regulator Bcl-X

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

BCL2L1

Gene ID

598

NCBI Gene ID

516966

UniProt

Q07817

Cellular Locus

Cytoplasmic|Endoplasmic reticulum|Mitochondria

Host

Mouse

Species Reactivity

Human,Mouse,Pig,Rat

Immunogen

A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.

Target Antigen

Bcl-x

Clonality

Monoclonal

Isotype

IgG2a

Clone

2H12

Conjugation

Purified, BSA-free

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Apoptosis, Cancer

Positive Control

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

27 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/865198/datasheet/bnum0751-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/865198/msds/bnum0751-50.pdf

CAS Number

9007-83-4

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