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Anti-AFP (Alpha Fetoprotein)(C2 + C3 + MBS-12), Biotin conjugate

Alpha-fetoprotein (AFP) is an oncofetal glycoprotein with a single chain of 70 kDa. This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Alpha-fetoglobulin; FETA; HPAFP

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

AFP

Gene ID

174

NCBI Gene ID

518808

UniProt

P02771

Cellular Locus

Cytoplasmic|Golgi apparatus

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant full-length human Alpha fetoprotein

Target Antigen

AFP | Alpha-fetoprotein

Clonality

Monoclonal

Isotype

IgG

Clone

C2 C3 MBS-12

Conjugation

Biotin

Disease

Tumor

Source

Animal

Applications

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified)

Validated Applications

FC,IF,IHC,FFPE

Field of Research

Cancer, Developmental biology

Positive Control

HepG2 cells. Fetal liver or hepatocellular carcinoma

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

70 kDa

Additionnal Information

Immunohistology formalin-fixed 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/864405/datasheet/bncb0810-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/864405/msds/bncb0810-100.pdf

CAS Number

9007-83-4

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