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Anti-Napsin A (Lung Adenocarcinoma Marker)(NAPSA/1238 + NAPSA/1239)

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Specifications

Synonyms

ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

NAPSA

Gene ID

9476

NCBI Gene ID

512843

UniProt

O96009

Cellular Locus

Secreted (extracellular)

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)

Target Antigen

Napsin-A

Clonality

Monoclonal

Isotype

IgG κ

Clone

NAPSA/1238 NAPSA/1239

Conjugation

Purified, BSA-free

Source

Animal

Applications

IHC, FFPE (verified) | WB (verified)

Validated Applications

IHC,FFPE,WB

Field of Research

Cancer, Proteases

Positive Control

Lung adenocarcinoma

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

37 kDa

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistochemistry (formalin-fixed): 1-2 ug/mL for 30 minutes at RT|Western blot: 1-2 ug/mL for 60 minutes at RT|Flow cytometry: 0.5-1 ug/million cells|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/863754/datasheet/bnum1254-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/863754/msds/bnum1254-50.pdf

CAS Number

9007-83-4

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