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Anti-Human Immunoglobulin Gamma (IgG) Heavy Chain(IG217 + IG266)

This MAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Gamma heavy chain(IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.

Product Specifications

Synonyms

G1m Marker; G2m Marker; G3m Marker; G4m Marker; HDC; Heavy Chain Disease Protein; Human Immunglobulin G; Ig gamma1/2/3/4 Chain C Region; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin Heavy Constant 1/2/3/4

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

IGHG

Gene ID

3500; 3501; 3502; 3503

NCBI Gene ID

510635

UniProt

P01857, P01859, P01860, P01861

Cellular Locus

Secreted (extracellular)

Host

Mouse

Species Reactivity

Human

Immunogen

Purified human IgG

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

IG217 IG266

Conjugation

Purified, BSA-free

Disease

Tumor

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Cancer, Immunology, Inflammation

Positive Control

293T, Raji or hPBL cells. Tonsil or Spleen

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

75 kDa

Additionnal Information

Flow cytometry: 1 ug/million cells|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 minutes at RT|Immunofluorescence: 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/863450/datasheet/bnum1262-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/863450/msds/bnum1262-50.pdf

CAS Number

9007-83-4

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