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Anti-Fibronectin (8th Type III Repeat)(568)

The epitope of this antibody is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Product Specifications

Synonyms

Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FN1

Gene ID

2335

NCBI Gene ID

203717

UniProt

P02751

Cellular Locus

Extracellular matrix

Host

Mouse

Species Reactivity

Human

Immunogen

High molecular weight proteins secreted by cultivated human fibroblasts

Target Antigen

Fibronectin

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

568

Conjugation

Purified, BSA-free

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Field of Research

Cell adhesion, Extracellular matrix

Positive Control

SW156 cells. Kidney.

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

220 kDa (monomer); 440 kDa (dimer)

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 minutes at RT|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/863222/datasheet/bnum0351-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/863222/msds/bnum0351-50.pdf

CAS Number

9007-83-4

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