Hoechst 33258

Hoechst 33258 is a blue to blue-green fluorescent live cell dye that can label DNA. Hoechst 33258 can specifically bind to the minor groove of DNA (and tends to bind to A/T-rich DNA), resulting in a significant increase in fluorescence intensity. Hoechst 33258 can cross the cell membrane and cause changes in DNA structure, such as G2/M phase arrest. Hoechst 33258 can bind to live or fixed cells, and the fluorescence intensity increases with increasing solution pH. As a DNA-specific probe, Hoechst 33258 can be used to detect DNA content, analyze cell cycle, etc. The excitation wavelength of Hoechst 33258 is 350-365 nm, and the emission wavelength is 460-490 nm[1][2][3].

Product Specifications

CAS Number

[23491-44-3]

Product Name Alternative

BisBenzimide H 33258; H 33258

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

DNA Alkylator/Crosslinker; DNA Stain; Fluorescent Dye

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage; Others

Applications

Cancer-programmed cell death

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Hoechst-33258.html

Purity

99.93

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

OC1=CC=C(C2=NC3=CC=C(C4=NC5=CC=C(N6CCN(C)CC6)C=C5N4)C=C3N2)C=C1

Molecular Formula

C25H24N6O

Molecular Weight

424.50

Precautions

H302, H315, H319, H335

References & Citations

[1]Stokke T, et al. Multiple binding modes for Hoechst 33258 to DNA. J Histochem Cytochem. 1985 Apr;33 (4) :333-8.|[2]Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through induction of apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22 (19) :6297-300.|[3]Pjura PE, et al. Binding of Hoechst 33258 to the minor groove of B-DNA. J Mol Biol. 1987 Sep 20;197 (2) :257-71.