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Anti-CD100 (Semaphorin-4D)(A8)

CD100 (also known as Semaphorin 4D), is a homodimeric protein comprised of 50 kDa subunits. It is expressed on resting T cells and weakly on other lymphocytes, and its expression is increased on B and T cells after stimulation. CD100 is a cell surface receptor that binds to CD72, Plexin B1 and Plexin B2. It promotes B cell aggregation, cell survival, and cell proliferation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

CD100; Semaphorin-4D; SEMA4D

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SEMA4D

Gene ID

10507

NCBI Gene ID

494406

UniProt

Q92854

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human,Monkey,Mouse

Immunogen

PHA stimulated human peripheral blood lymphocytes.

Target Antigen

CD100 | Semaphorin-4D

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

A8

Conjugation

Purified, with BSA

Source

Animal

Applications

Flow, surface (published) | ELISA (published) | WB (published)

Validated Applications

FC,ELISA,WB

Field of Research

Immunology, Neuroscience, Signal transduction

Positive Control

Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

50 kDa (Monomer)

Additionnal Information

Functional studies: Order Ab without azide; Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1996) 93:11780-11785. (Flow) PLoS One (2007) 2(9):e818. (Flow, WB, ELISA)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/862270/datasheet/bnub0218-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/862270/msds/bnub0218-500.pdf

CAS Number

9007-83-4

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