DiSC2(5) (3, 3'-diethylthiadicarboCyanine, iodide)

DiSC₂(5) (3,3'-Diethylthiadicarbocyanine, iodide) binds to DNA-DNA or PNA-DNA duplexes with a large blue-shift of the absorption spectrum. Binding of the dye to PNA-DNA hybrid, in particular, blue-shifts the dye absorption spectrum by as much as 115 nm, offering a visual method to detect nucleic acid hybridization. <ul> <li> lamba;_Abs (pH 7.5 buffer) = 646 nm (free dye); <em>~</em>586 nm (dsDNA); <em>~</em>531 nm (PNA-DNA)</li> <li>Dark solid soluble in DMSO or MeOH</li> <li>Store at 4°C</li> <li>C₂₃H₂₃IN₂S₂</li> <li>MW: 518.48</li> </ul>