Caspase 9 Activity Assay Kit (Colorimetric Method)

Product Specifications

Detection Method

Colorimetric method

Assay Principle

Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of apoptosis, thereby promoting subsequent apoptotic signals. The activation of caspase 9 can be regulated by phosphorylation. This caspase 9 activity assay kit is based on caspase 9 that can catalyze the substrate Ac-LEHD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 9 can be calculated by determining the absorbance at 405 nm.

Sample Type

Cell and tissue samples

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S0141813025006841

Citation 02

https://www.sciencedirect.com/science/article/pii/S0925521423003162

Citation 03

https://www.sciencedirect.com/science/article/pii/S0925521423001369

Citation 04

https://www.sciencedirect.com/science/article/pii/S0925521422002678

Citation 05

https://www.sciencedirect.com/science/article/pii/S0925521424005106

Citation 06

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c09673

Citation 07

https://www.sciencedirect.com/science/article/pii/S1773224724008281

Citation 08

https://link.springer.com/article/10.1007/s12035-025-04810-y

Citation 09

https://www.nature.com/articles/s41598-025-96788-9

Citation 10

https://link.springer.com/article/10.1007/s00210-025-04355-w

Citation 11

https://www.sciencedirect.com/science/article/pii/S1756464624001713

Citation 12

https://www.sciencedirect.com/science/article/pii/S0039128X22001908

Detection Instrument

Spectrophotometer; Microplate reader

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

YP-W11265-01 48 Tests

Human neuropeptide Y (NPY) ELISA Kit

Ask

View Details

YP-W11265-02 96 Tests

Human neuropeptide Y (NPY) ELISA Kit

Ask

View Details

MBS9004312 Inquire

Rabbit anti-Oryza sativa subsp. japonica (Rice) Os10g0490500 Polyclonal Antibody

Ask

View Details

157850 96 Tests

Interleukin 1 Epsilon (IL1e) BioAssayTM ECL ELISA Kit (Human)

Ask

View Details

MBS1027551-01 0.02 mg (E-Coli)

Recombinant Aspergillus niger Actin cytoskeleton-regulatory complex protein end3 (end3)

Ask

View Details

MBS1027551-02 0.02 mg (Yeast)

Recombinant Aspergillus niger Actin cytoskeleton-regulatory complex protein end3 (end3)

Ask

View Details