CKI-7

CKI-7 is a potent and ATP-competitive casein kinase 1 (CK1) inhibitor with an IC50 of 6 μM and a Ki of 8.5 μM. CKI-7 is a selective Cdc7 kinase inhibitor. CKI-7 also inhibits SGK, ribosomal S6 kinase-1 (S6K1) and mitogen- and stress-activated protein kinase-1 (MSK1) . CKI-7 has a much weaker effect on casein kinase II and other protein kinases[1][2][3][4].

Product Specifications

CAS Number

[1177141-67-1]

UNSPSC

12352005

Hazard Statement

H301

Target

Casein Kinase; CDK; Ribosomal S6 Kinase (RSK) ; SGK

Type

Reference compound

Related Pathways

Cell Cycle/DNA Damage; MAPK/ERK Pathway; Metabolic Enzyme/Protease; Stem Cell/Wnt

Applications

Cancer-Kinase/protease

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/cki7.html

Purity

99.97

Solubility

DMSO : 5 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 10 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=S(C1=CC=C(Cl)C2=C1C=NC=C2)(NCCN)=O.[H]Cl.[H]Cl

Molecular Formula

C11H14Cl3N3O2S

Molecular Weight

358.67

Precautions

H301

References & Citations

[1] Osakada F, et al. In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction. J Cell Sci. 2009 Sep 1;122 (Pt 17) :3169-79.|[2]Mark G. Frattini, et al. Small Molecule Inhibition of Cdc7, a Key Cell Cycle Regulator and Novel Therapeutic Target, Successfully Inhibits Leukemia Cell Growth in Vitro and in Vivo. Blood (2008) 112 (11) : 2668.|[3]Chijiwa T, et al. A newly synthesized selective casein kinase I inhibitor, N- (2-aminoethyl) -5-chloroisoquinoline-8-sulfonamide, and affinity purification of casein kinase I from bovine testis. J Biol Chem. 1989 Mar 25;264 (9) :4924-7.|[4]Rena G, et al. D4476, a cell-permeant inhibitor of CK1, suppresses the site-specific phosphorylation and nuclear exclusion of FOXO1a. EMBO Rep. 2004 Jan;5 (1) :60-5.