Rabbit anti Human Ceramide Kinase (rProtein-Full Length)
Product Specifications
Background
Sphingolipids and their metabolic products, including ceramide, sphingosine, and sphingosine-1-phosphate (S1P),1 are important signaling molecules in many biological processes. Ceramide has been is one of the key elements regulating apoptotic responses. Ceramide has been reported as a regulator of several protein kinases and phosphatases, including ceramide-activated protein kinase, protein phosphatase, and protein kinase C. Sphingosine, a metabolite of ceramide, might also play a role in mitochondria-mediated apoptosis. Sphingosine inhibits several protein kinases, including protein kinase C and Ca2 -calmodulin-dependent kinase II. S1P regulates many biological processes, including mitogenesis, differentiation, migration, and suppression of apoptosis.
Synonyms
Acylsphingosine kinase, hCERK, Lipid kinase 4, LK4
UniProt
Q8TCT0
Host
Rabbit
Species Reactivity
Human
Conjugation
Unconjugated
Type
Polyclonal Antibody
Source
Rabbits were immunized with recombinant full-length Human ceramide kinase protein
Applications
ELISA, WB
Field of Research
Non-protein Kinase
Assay Principle
Antibody can be used for Western blotting (1-10µg/mL). Can also be used for ELISA. Optimal concentration should be evaluated by serial dilutions.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Lamour NF, Chalfant CE. ‘Ceramide-1-phosphate: The missing link in eicosanoid biosynthesis and inflammation.’ Mol Interv. 2005 Dec;5(6):358-67.
2.Kim JW, Inagaki Y, Mitsutake S, Maezawa N, Katsumura S, Ryu YW, Park CS, Taniguchi M, Igarashi Y. ‘Suppression of mast cell degranulation by a novel ceramide kinase inhibitor, the F-12509A olefin isomer K1.’ Biochim Biophys Acta. 2005 Dec 30;1738(1-3):82-90. Epub 2005 Nov 14. _x000D_
3. Van Overloop H, Gijsbers S, Van Veldhoven PP. ‘Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies.’
J Lipid Res. 2006 Feb;47(2):268-83. Epub 2005 Nov 3._x000D_
4. Chalfant CE, Spiegel S. ‘Sphingosine 1-phosphate and ceramide 1-phosphate: expanding roles in cell signaling.’ J Cell Sci. 2005 Oct 15;118(Pt 20):4605-12. Review. _x000D_
5. Mitsutake S, Igarashi Y. ‘Calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium sensor.’ J Biol Chem. 2005 Dec 9;280(49):40436-41. Epub 2005 Oct 3. _x000D_
6. Wijesinghe DS, Massiello A, Subramanian P, Szulc Z, Bielawska A, Chalfant CE. ‘Substrate specificity of Human ceramide kinase.’
J Lipid Res. 2005 Dec;46(12):2706-16. Epub 2005 Sep 18. _x000D_
7.Kim TJ, Mitsutake S, Kato M, Igarashi Y. ‘The leucine 10 residue in the pleckstrin homology domain of ceramide kinase is crucial for its catalytic activity.’ FEBS Lett. 2005 Aug 15;579(20):4383-8.
Shipping Conditions
Ambient Temperature, freeze upon arrival
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
WB
CAS Number
9007-83-4
Curated Selection
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