YO-PRO-1

YO-PRO-1 is a non-cell-membrane-penetrating green fluorescent dye. YO-PRO-1 cannot penetrate normal cell membranes but can penetrate the membranes of apoptotic and necrotic cells. YO-PRO-1 has a high affinity for DNA. When it is not bound to DNA, it emits almost no fluorescence, and when it binds to DNA, it can emit green fluorescence (Ex/Em: 488/530±30 nM) . YO-PRO-1 is often used together with Propidium Iodide (HY-D0815) to analyze and identify apoptotic and necrotic cells[1][2].

Product Specifications

CAS Number

[152068-09-2]

UNSPSC

12171500

Hazard Statement

H301, H315, H319

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/yo-pro-1.html

Purity

98.20

Solubility

10 mM in DMSO

Smiles

C[N+](CCC[N+]1=C2C=CC=CC2=C(/C=C3OC4=CC=CC=C4N\3C)C=C1)(C)C.[I-].[I-]

Molecular Formula

C24H29I2N3O

Molecular Weight

629.32

Precautions

H301, H315, H319

References & Citations

[1]Boffa DJ, et al. Measurement of apoptosis of intact human islets by confocal optical sectioning and stereologic analysis of YO-PRO-1-stained islets. Transplantation. 2005 Apr 15;79 (7) :842-5.|[2]Fujisawa S, et al. Evaluation of YO-PRO-1 as an early marker of apoptosis following radiofrequency ablation of colon cancer liver metastases. Cytotechnology. 2014 Mar;66 (2) :259-73.